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RIP (phospho Tyr384) antibody

Orthogonal
Anti-RIP (phospho Tyr384) antibody used in ELISA (ELISA). GTX03682
Anti-RIP (phospho Tyr384) antibody used in IHC (Paraffin sections) (IHC-P). GTX03682
Anti-RIP (phospho Tyr384) antibody used in Western Blot (WB). GTX03682
Anti-RIP (phospho Tyr384) antibody used in IHC (Paraffin sections) (IHC-P). GTX03682

Cat. No. GTX03682

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all RIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Observed MW

76 kDa.

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human RIPK1 (Accession Q13546), corresponding to amino acid residues E371-T395.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor interacting serine/threonine kinase 1 , IMD57 , RIP , RIP-1 , RIP1

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-RIP (phospho Tyr384) antibody used in ELISA (ELISA). GTX03682

GTX03682 ELISA Image

Orthogonal

ELISA analysis of phospho- and non phospho- immunogen peptide using GTX03682 RIP (phospho Tyr384) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-RIP (phospho Tyr384) antibody used in IHC (Paraffin sections) (IHC-P). GTX03682

GTX03682 IHC-P Image

IHC-P analysis of human liver tissue using GTX03682 RIP (phospho Tyr384) antibody.
Dilution : 1:100
Antigen retrieval : Heat mediated citrate buffer

Anti-RIP (phospho Tyr384) antibody used in Western Blot (WB). GTX03682

GTX03682 WB Image

WB analysis of 293 lysates using GTX03682 RIP (phospho Tyr384) antibody. Lane 1 was treated with the blocking peptide.

Anti-RIP (phospho Tyr384) antibody used in IHC (Paraffin sections) (IHC-P). GTX03682

GTX03682 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX03682 RIP (phospho Tyr384) antibody.
Dilution : 1:100
Antigen retrieval : Heat mediated citrate buffer

REFERENCE

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REVIEW

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Package List Price ($)
$ 419