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RIP2 antibody [C1C3]

KO/KD
Anti-RIP2 antibody [C1C3] used in Western Blot (WB). GTX113045
Anti-RIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113045
Anti-RIP2 antibody [C1C3] used in Western Blot (WB). GTX113045

Cat. No. GTX113045

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RIP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Molt-4 , HeLa

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RIP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951724

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor interacting serine/threonine kinase 2 , CARD3 , CARDIAK , CCK , GIG30 , RICK , RIP2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RIP2 antibody [C1C3] used in Western Blot (WB). GTX113045

GTX113045 WB Image

KO/KD

Wild-type (WT) and RIP2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP2 antibody [C1C3] (GTX113045) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113045

GTX113045 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using RIP2(GTX113045) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RIP2 antibody [C1C3] used in Western Blot (WB). GTX113045

GTX113045 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX113045 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169