Wild-type (WT) and RIP2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP2 antibody [N3C3] (GTX105939) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using RIP2(GTX105939) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using RIP2(GTX105939) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
10% SDS PAGE
GTX105939 diluted at 1:2000