GeneTex
United States (US)

RIP2 antibody [N3C3]

Anti-RIP2 antibody [N3C3] used in Western Blot (WB). GTX105939
Anti-RIP2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX105939
Anti-RIP2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105939
Anti-RIP2 antibody [N3C3] used in Western Blot (WB). GTX105939

Cat No. GTX105939

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

HeLa , 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Receptor Interacting Serine/Threonine Kinase 2,Card3,Cardiak,Cck,Gig30,Rick,Rip2,Ripk2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-RIP2 antibody [N3C3] used in Western Blot (WB). GTX105939

GTX105939 WB Image

Wild-type (WT) and RIP2 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP2 antibody [N3C3] (GTX105939) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The CRISPR knockout cell line was generated by EdiGene Inc.

Anti-RIP2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX105939

GTX105939 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using RIP2(GTX105939) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RIP2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105939

GTX105939 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using RIP2(GTX105939) antibody at 1:200 dilution.

Anti-RIP2 antibody [N3C3] used in Western Blot (WB). GTX105939

GTX105939 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
10% SDS PAGE
GTX105939 diluted at 1:2000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169