GeneTex
United States (US)

RIP2 antibody

RIP2-antibody_ICCIF_GTX28427-1_18121411_672.jpg
RIP2-antibody_WB_GTX28427-1_18121411_767.jpg

Cat No. GTX28427

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For WB: Use at a dilution of 1:500. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

61 kDa. ( Note )

Positive Control

K562 or 3T3 whole cell lysate
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - amino acids 508-522 of RIP2

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Receptor Interacting Serine/Threonine Kinase 2,Card3,Cardiak,Cck,Gig30,Rick,Rip2,Ripk2

Background

Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designatedRICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively, (1-3). RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-kB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of pro-inflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues (1).

Database

Research Area

DATA IMAGES
RIP2-antibody_ICCIF_GTX28427-1_18121411_672.jpg

GTX28427 ICC/IF Image

Immunocytochemical staining of RIP2 in K562 cells with RIP2 antibody (GTX28427) at 5μg/ml.

RIP2-antibody_WB_GTX28427-1_18121411_767.jpg

GTX28427 WB Image

Western blot analysis of RIP2 in A431 cell lysate with RIP2 antibody (GTX28427) at (A) 1 and (B) 2μg/ml.

Package List Price ($)
$ 329