GeneTex
United States (US)

RIP2 antibody

RICK-antibody_ICCIF_GTX28428-1_18121411_678.jpg
RICK-antibody_WB_GTX28428-1_18121411_470.jpg

Cat No. GTX28428

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

For WB: Use at a dilution of 1:900 - 1:1,100. Optimal concentrations/dilutions should be determined by the researcher.

Calculated MW

61 kDa. ( Note )

Positive Control

Whole cell lysate from HeLa cells

Specificity/Sensitivity

Human RICK
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.4 with 0.02% sodium azide as preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 11-30 of Human RICK.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Receptor Interacting Serine/Threonine Kinase 2,Card3,Cardiak,Cck,Gig30,Rick,Rip2,Ripk2

Background

Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-threonine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designatedRICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively, (1-3). RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-kB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of pro-inflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues (1).

Database

Research Area

DATA IMAGES
RICK-antibody_ICCIF_GTX28428-1_18121411_678.jpg

GTX28428 ICC/IF Image

Immunocytochemical staining of RIP2 in A431 cells with RIP2 antibody (GTX28428) at 10μg/ml.

RICK-antibody_WB_GTX28428-1_18121411_470.jpg

GTX28428 WB Image

Western blot analysis of RIP2 in A431 (A) and K562 (K) whole cell lysate with RIP2 antibody (GTX28428) at 1 g/ml.

Package List Price ($)
$ 369