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RIP3 antibody

Anti-RIP3 antibody used in Western Blot (WB). GTX131188
Anti-RIP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131188
Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188
Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188
Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188

Cat No. GTX131188

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

THP-1

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886437

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

receptor interacting serine/threonine kinase 3 , RIP3

Cellular Localization

Cytoplasm

Background

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RIP3 antibody used in Western Blot (WB). GTX131188

GTX131188 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with RIP3 antibody (GTX131188) diluted at 1:1000 and competitor's antibody (CPA4203) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-RIP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131188

GTX131188 ICC/IF Image

RIP3 antibody detects RIP3 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RIP3 stained by RIP3 antibody (GTX131188) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188

GTX131188 IHC-P Image

RIP3 antibody detects RIP3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
RIP3 stained by RIP3 antibody (GTX131188) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188

GTX131188 IHC-P Image

RIP3 antibody detects RIP3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
RIP3 stained by RIP3 antibody (GTX131188) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188

GTX131188 IHC-P Image

RIP3 antibody detects RIP3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RIP3 stained by RIP3 antibody (GTX131188) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169