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RIP3 antibody

Comparison
Anti-RIP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131188
Anti-RIP3 antibody used in Western Blot (WB). GTX131188
Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188
Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188
Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188

Cat. No. GTX131188

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all RIP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

THP-1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886437

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor interacting serine/threonine kinase 3 , RIP3

Cellular Localization

Cytoplasm

Background

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RIP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131188

GTX131188 ICC/IF Image

RIP3 antibody detects RIP3 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RIP3 stained by RIP3 antibody (GTX131188) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RIP3 antibody used in Western Blot (WB). GTX131188

GTX131188 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with RIP3 antibody (GTX131188) diluted at 1:1000 and competitor's antibody (CPA4203) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188

GTX131188 IHC-P Image

RIP3 antibody detects RIP3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RIP3 stained by RIP3 antibody (GTX131188) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188

GTX131188 IHC-P Image

RIP3 antibody detects RIP3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
RIP3 stained by RIP3 antibody (GTX131188) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131188

GTX131188 IHC-P Image

RIP3 antibody detects RIP3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
RIP3 stained by RIP3 antibody (GTX131188) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

H Cui et al. Research Square 2023; TNF-α promotes osteocyte necroptosis by upregulating TLR4 in postmenopausal osteoporosis
Application : IHC-P
Engelmann C et al. Sci Rep 2020; 10 (1) : 389 Recombinant Alkaline Phosphatase Prevents Acute on Chronic Liver Failure.
Application : IHC-P
Reactivity : Rat
You BJ et al. Sci Rep 2019; 9 (1) : 3306 Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169