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RIT2 antibody [3F4]

Overexpression
Anti-RIT2 antibody [3F4] used in Western Blot (WB). GTX83710
Anti-RIT2 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX83710
Anti-RIT2 antibody [3F4] used in Flow cytometry (FACS). GTX83710
Anti-RIT2 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX83710
Anti-RIT2 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX83710
Anti-RIT2 antibody [3F4] used in Western Blot (WB). GTX83710
Anti-RIT2 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX83710

Cat. No. GTX83710

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F4

Isotype

 IgG2a

Application

 WB, IHC-P, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($489)
See all RIT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human RIT2 (NP_002921) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras like without CAAX 2 , RIBA , RIN , ROC2

Cellular Localization

Nucleus,Cell membrane

Background

Binds and exchanges GTP and GDP.

Database

Research Area

DATA IMAGES

Anti-RIT2 antibody [3F4] used in Western Blot (WB). GTX83710

GTX83710 WB Image

WB analysis of various cell lines using GTX83710 RIT2 antibody [3F4].
Loading : 35 ug per lane

Anti-RIT2 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX83710

GTX83710 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83710 RIT2 antibody [3F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RIT2 antibody [3F4] used in Flow cytometry (FACS). GTX83710

GTX83710 FACS Image

FACS analysis of HEK293T cells transfected with either RIT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83710 RIT2 antibody [3F4].

Anti-RIT2 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX83710

GTX83710 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83710 RIT2 antibody [3F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RIT2 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX83710

GTX83710 IHC-P Image

IHC-P analysis of human breast tissue using GTX83710 RIT2 antibody [3F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RIT2 antibody [3F4] used in Western Blot (WB). GTX83710

GTX83710 WB Image

Overexpression

WB analysis of HEK293T cells transfected with RIT2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83710 RIT2 antibody [3F4].
Loading : 5 ug per lane

Anti-RIT2 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX83710

GTX83710 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX83710 RIT2 antibody [3F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489