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RKIP antibody [SC58-09]

Recombinant antibody Orthogonal
Anti-RKIP antibody [SC58-09] used in Western Blot (WB). GTX01163
Anti-RKIP antibody [SC58-09] used in Western Blot (WB). GTX01163
Anti-RKIP antibody [SC58-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01163
Anti-RKIP antibody [SC58-09] used in IHC (Paraffin sections) (IHC-P). GTX01163
Anti-RKIP antibody [SC58-09] used in IHC (Paraffin sections) (IHC-P). GTX01163
Anti-RKIP antibody [SC58-09] used in IHC (Paraffin sections) (IHC-P). GTX01163

Cat. No. GTX01163

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 SC58-09

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all RKIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylethanolamine binding protein 1 , HCNP , HCNPpp , HEL210 , HELS34 , HELS96 , PBP , PEBP , PEBP1 , RKIP

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-RKIP antibody [SC58-09] used in Western Blot (WB). GTX01163

GTX01163 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RKIP antibody [SC58-09] (GTX01163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-RKIP antibody [SC58-09] used in Western Blot (WB). GTX01163

GTX01163 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RKIP antibody [SC58-09] (GTX01163) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RKIP antibody [SC58-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01163

GTX01163 ICC/IF Image

ICC/IF analysis of PFA-fixed PC-3M cells using GTX01163 RKIP antibody [SC58-09].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-RKIP antibody [SC58-09] used in IHC (Paraffin sections) (IHC-P). GTX01163

GTX01163 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX01163 RKIP antibody [SC58-09].

Anti-RKIP antibody [SC58-09] used in IHC (Paraffin sections) (IHC-P). GTX01163

GTX01163 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01163 RKIP antibody [SC58-09].

Anti-RKIP antibody [SC58-09] used in IHC (Paraffin sections) (IHC-P). GTX01163

GTX01163 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01163 RKIP antibody [SC58-09].

REFERENCE

Application Reference

Mandal JP et al. Free Radic Biol Med 2021; 163 : 69-87 PKCδ mediates mitochondrial ROS generation and oxidation of HSP60 to relieve RKIP inhibition on MAPK pathway for HCC progression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399