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RNA polymerase beta antibody [8RB13]

Cat. No. GTX12087

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8RB13

Isotype

 IgG2b

Application

 WB, IP, ELISA

Reactivity

 E. coli, Bacteria
Package
50 μg ($399)
See all RNA polymerase beta products

APPLICATION

Application Note

WB: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Reacts with beta subunit of RNA polymerase of E. coli

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

RNA polymerase from Escherichia coli.

Purification

Affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nuclear

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Research Area

REFERENCE

Application Reference

Posp??il J et al. Commun Biol 2024; 7 (1) : 46 σ(E) of Streptomyces coelicolor can function both as a direct activator or repressor of transcription.

Clone Reference

Stalder ES et al. Protein Expr Purif 2011; 77 (1):26-33 The epitope for the polyol-responsive monoclonal antibody 8RB13 is in the flap-domain of the beta-subunit of bacterial RNA polymerase and can be used as an epitope tag for immunoaffinity chromatography.
Application : WB
Reactivity : E. coli
Bergendahl V et al. Protein Expr Purif 2003; 31 (1):155-60 A cross-reactive polyol-responsive monoclonal antibody useful for isolation of core RNA polymerase from many bacterial species.
Application : WB
Reactivity : E. coli
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Package List Price ($)
$ 399
Custom Conjugations