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RNAse H2A antibody

Orthogonal
Anti-RNAse H2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115433
Anti-RNAse H2A antibody used in Western Blot (WB). GTX115433

Cat. No. GTX115433

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RNAse H2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa , HeLa NE

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNAse H2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885363

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonuclease H2 subunit A , AGS4 , JUNB , RNASEHI , RNHIA , RNHL , THSD8

Cellular Localization

Nucleus

Background

Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RNAse H2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115433

GTX115433 ICC/IF Image

RNAse H2A Peptide detects RNASEH2A protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RNASEH2A protein stained by RNAse H2A Peptide (GTX115433) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RNAse H2A antibody used in Western Blot (WB). GTX115433

GTX115433 WB Image

Orthogonal

RNASEH2A antibody detects RNASEH2A protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNASEH2A antibody (GTX115433) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169