GeneTex
United States (US)

RNAse H2A antibody

Orthogonal
GTX115433_41206_20150525_IFA_18121410_932.jpg
GTX115433_41206_WB_Fraction_18121410_308.jpg

Cat No. GTX115433

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa , HeLa NE

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.82mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNAse H2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RNASEHI Antibody, RNHIA Antibody, JUNB Antibody, AGS4 Antibody, ribonuclease H2, subunit A Antibody, RNHL Antibody

Cellular Localization

Nucleus

Background

Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli. [provided by RefSeq]
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Database

DATA IMAGES
GTX115433_41206_20150525_IFA_18121410_932.jpg

GTX115433 ICC/IF Image

RNAse H2A Peptide detects RNASEH2A protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RNASEH2A protein stained by RNAse H2A Peptide (GTX115433) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX115433_41206_WB_Fraction_18121410_308.jpg

GTX115433 WB Image

Orthogonal

RNASEH2A antibody detects RNASEH2A protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNASEH2A antibody (GTX115433) diluted by 1:1000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169