GeneTex

  • Country / Location Selection

United States (US)

RNAse H2A antibody

Anti-RNAse H2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85020
Anti-RNAse H2A antibody used in Western Blot (WB). GTX85020
Anti-RNAse H2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85020

Cat. No. GTX85020

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all RNAse H2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A.The immunogen is located within amino acids 190 - 240 of RNAse H2A.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10725988

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonuclease H2 subunit A , AGS4 , JUNB , RNASEHI , RNHIA , RNHL , THSD8

Cellular Localization

Nucleus

Background

Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.

Database

Research Area

DATA IMAGES

Anti-RNAse H2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85020

GTX85020 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85020 RNAse H2A antibody.
Working concentration : 2 μg/ml

Anti-RNAse H2A antibody used in Western Blot (WB). GTX85020

GTX85020 WB Image

WB analysis of HeLa cell lysate using GTX85020 RNAse H2A antibody.
Working concentration : 1 μg/ml

Anti-RNAse H2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85020

GTX85020 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85020 RNAse H2A antibody.
Working concentration : 5 μg/ml

REFERENCE

Application Reference

Ghodgaonkar MM et al. Mol Cell 2013; 50 (3) : 323-32 Ribonucleotides misincorporated into DNA act as strand-discrimination signals in eukaryotic mismatch repair.
Application : WB
Reactivity : Human, Mouse
Submit a Reference

REVIEW

There are currently no reviews for RNAse H2A antibody (GTX85020). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399