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RNF128 antibody, C-term

KO/KD
Anti-RNF128 antibody, C-term used in Western Blot (WB). GTX46906
Anti-RNF128 antibody, C-term used in Western Blot (WB). GTX46906

Cat. No. GTX46906

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all RNF128 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 , mouse CD4 T cells

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human RNF128

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 128 , GRAIL

Cellular Localization

Cytoplasm, cytoskeleton

Background

The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RNF128 antibody, C-term used in Western Blot (WB). GTX46906

GTX46906 WB Image

KO/KD

WB analysis of mouse CD4 T cells using GTX46906 RNF128 antibody at 1:2400.
Lane:
1: CD4 T cell lysate from WT mice
2: CD4 T cell lysate from WT mice treated with anti-CD3/CD28
3: CD4 T cell lysate from WT mice treated with FK506
4: CD4 T cell lysate from WT mice treated with anti-CD3/CD28 and FK506
5: CD4 T cell lysate from TRPV1 KO mice
6: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28
7: CD4 T cell lysate from TRPV1 KO mice treated with FK506
8: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28 and FK506.

Anti-RNF128 antibody, C-term used in Western Blot (WB). GTX46906

GTX46906 WB Image

WB analysis of HepG2 cells using GTX46906 RNF128 antibody at 1.25μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399