GeneTex
United States (US)

RNF13 antibody [C3], C-term

GTX106435_WB_19011506_807.jpg
GTX106435_40450_IFA_19011506_391.jpg
GTX106435_IHC_19011506_283.jpg

Cat No. GTX106435

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RNF13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ93817 antibody, MGC13689 antibody, RZF antibody, RNF13 antibody, E3 ubiquitin-protein ligase RNF13 antibody, RING zinc finger protein antibody, ring finger protein 13 antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. The specific function of this gene has not yet been determined. Alternatively spliced transcript variants that encode the same protein have been reported. A pseudogene, which is also located on chromosome 3, has been defined for this gene. [provided by RefSeq]
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Database

DATA IMAGES
GTX106435_WB_19011506_807.jpg

GTX106435 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX106435 diluted at 1:1000

GTX106435_40450_IFA_19011506_391.jpg

GTX106435 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using RNF13(GTX106435) antibody at 1:500 dilution.

GTX106435_IHC_19011506_283.jpg

GTX106435 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using RNF13(GTX106435) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169