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RNF14 antibody [N1C1]

Anti-RNF14 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112193
Anti-RNF14 antibody [N1C1] used in Western Blot (WB). GTX112193

Cat No. GTX112193

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

IMR32 , U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNF14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174092

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ring finger protein 14 , ARA54 , HFB30 , HRIHFB2038 , TRIAD2

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RNF14 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112193

GTX112193 ICC/IF Image

RNF14 antibody [N1C1] detects RNF14 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RNF14 protein stained by RNF14 antibody [N1C1] (GTX112193) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RNF14 antibody [N1C1] used in Western Blot (WB). GTX112193

GTX112193 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX112193 diluted at 1:1000

REFERENCE

There are currently no references for RNF14 antibody [N1C1] (GTX112193). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RNF14 antibody [N1C1] (GTX112193). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169