RNF144B antibody [9H10]

Cat. No. GTX83704

Host	Mouse
Clonality	Monoclonal
Clone Name	9H10
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($519)

See all RNF144B products

Datasheet
Document

View product citations for antibody GTX83704 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:50-1:100
IHC-P	1:50
FACS	1:100

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 144B , IBRDC2 , PIR2 , bA528A10.3 , p53RFP

Cellular Localization

Cytoplasm

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death | But, it is not an anti-apoptotic protein per se.

Database

Gene ID: 255488 RNF144B
UniProt: Q7Z419 RNF144B

Research Area

DATA IMAGES

GTX83704 FACS Image

FACS analysis of HEK293T cells transfected with either RNF144B plasmid(Red) or empty vector control plasmid(Blue) using GTX83704 RNF144B antibody [9H10].

GTX83704 FACS Image

FACS analysis of HeLa cells using GTX83704 RNF144B antibody [9H10].
Red : Primary antibody
Blue : Negative control antibody

GTX83704 FACS Image

FACS analysis of Jurkat cells using GTX83704 RNF144B antibody [9H10].
Red : Primary antibody
Blue : Negative control antibody

GTX83704 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83704 RNF144B antibody [9H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83704 IHC-P Image

IHC-P analysis of human ovary tissue using GTX83704 RNF144B antibody [9H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83704 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with RNF144B plasmid using GTX83704 RNF144B antibody [9H10].

GTX83704 IHC-P Image

IHC-P analysis of human colon tissue using GTX83704 RNF144B antibody [9H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83704 WB Image

WB analysis of HEK293T cells transfected with RNF144B plasmid (Right) or empty vector (Left) for 48 hrs using GTX83704 RNF144B antibody [9H10].
Loading : 5 ug per lane

GTX83704 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83704 RNF144B antibody [9H10].

GTX83704 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83704 RNF144B antibody [9H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83704 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83704 RNF144B antibody [9H10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

RNF144B antibody [9H10]

Cat. No. GTX83704

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83704 FACS Image

GTX83704 FACS Image

GTX83704 FACS Image

GTX83704 IHC-P Image

GTX83704 IHC-P Image

GTX83704 ICC/IF Image

GTX83704 IHC-P Image

GTX83704 WB Image

GTX83704 ICC/IF Image

GTX83704 IHC-P Image

GTX83704 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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