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RNF168 antibody

Orthogonal Overexpression
Anti-RNF168 antibody used in Western Blot (WB). GTX129617
Anti-RNF168 antibody used in Western Blot (WB). GTX129617

Cat. No. GTX129617

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RNF168 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

human RNF168-transfected 293T cells , MDA-MB-231 nuclear extract

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNF168. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886056

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 168 , hRNF168

Cellular Localization

Nucleus

Background

The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RNF168 antibody used in Western Blot (WB). GTX129617

GTX129617 WB Image

Overexpression

RNF168 antibody detects RNF168 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human RNF168-transfected 293T cells
7.5 % SDS-PAGE
RNF168 antibody (GTX129617) dilution: 1:5000

Anti-RNF168 antibody used in Western Blot (WB). GTX129617

GTX129617 WB Image

Orthogonal

RNF168 antibody detects RNF168 protein by western blot analysis.
A. 30 μg MDA-MB-231 whole cell extract
B. 30 μg MDA-MB-231 nuclear extract
7.5 % SDS-PAGE
RNF168 antibody (GTX129617) dilution: 1:1000

REFERENCE

Application Reference

Heyza JR et al. Elife 2023; 12 Systematic analysis of the molecular and biophysical properties of key DNA damage response factors.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169