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RNF185 antibody [N1C3]

Anti-RNF185 antibody [N1C3] used in Western Blot (WB). GTX122691

Cat No. GTX122691

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

human RNF185-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNF185. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173926

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ring finger protein 185

Cellular Localization

Membrane; Multi-pass membrane protein

Background

E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).

Database

Research Area

DATA IMAGES

Anti-RNF185 antibody [N1C3] used in Western Blot (WB). GTX122691

GTX122691 WB Image

RNF185 antibody detects RNF185 protein by western blot analysis. Non-transfected (-) and RNF185-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNF185 antibody (GTX122691) at a dilution of 1:5000.

REFERENCE

There are currently no references for RNF185 antibody [N1C3] (GTX122691). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RNF185 antibody [N1C3] (GTX122691). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf