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RNF2 antibody

Anti-RNF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48486
Anti-RNF2 antibody used in Western Blot (WB). GTX48486

Cat No. GTX48486

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat, Bovine, Dog, Chicken, Chimpanzee, Orangutan
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:300
ELISA 1:5000 - 1:25000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 189-201 of human RNF2 protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ring finger protein 2 , BAP-1 , BAP1 , DING , HIPI3 , RING1B , RING2

Cellular Localization

Nucleus

Background

RING1B (also known as BAP1, DING, Polycomb-M33 interacting protein Ring1B, Ring finger protein 1b, Ring finger protein 2 and RNF2) is one of the PcG proteins. The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity.

Database

Research Area

DATA IMAGES

Anti-RNF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48486

GTX48486 ICC/IF Image

Immunofluorescence Microscopy of Goat anti-RING1B antibody (GTX48486). Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25ºC. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25ºC. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.

Anti-RNF2 antibody used in Western Blot (WB). GTX48486

GTX48486 WB Image

Western blot using GeneTex's Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.

REFERENCE

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SDS
Sodium Azide.pdf