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RNF2 antibody

Anti-RNF2 antibody used in Immunoprecipitation (IP). GTX54707
Anti-RNF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54707
Anti-RNF2 antibody used in ChIP assay (ChIP assay). GTX54707
Anti-RNF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54707
Anti-RNF2 antibody used in Western Blot (WB). GTX54707
Anti-RNF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54707

Cat. No. GTX54707

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RNF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
ChIP assay 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RNF2 (NP_009143.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 2 , BAP-1 , BAP1 , DING , HIPI3 , RING1B , RING2

Cellular Localization

Nucleus

Background

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RNF2 antibody used in Immunoprecipitation (IP). GTX54707

GTX54707 IP Image

IP analysis of 293T cell lysate using GTX54707 RNF2 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-RNF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54707

GTX54707 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX54707 RNF2 antibody.
Dilution : 1:100

Anti-RNF2 antibody used in ChIP assay (ChIP assay). GTX54707

GTX54707 ChIP assay Image

ChIP analysis of HeLa cell lysate using GTX54707 RNF2 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-RNF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54707

GTX54707 IHC-P Image

IHC-P analysis of rat lung tissue using GTX54707 RNF2 antibody.
Dilution : 1:100

Anti-RNF2 antibody used in Western Blot (WB). GTX54707

GTX54707 WB Image

WB analysis of various sample lysates using GTX54707 RNF2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RNF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54707

GTX54707 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX54707 RNF2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399