GeneTex
United States (US)

RNF20 antibody

GTX31540_1150_IHC-P_20180221_18121410_729.jpg
GTX31540_2725_WB_20180221_18121410_249.jpg
GTX31540_1151_IHC-P_20180221_18121410_801.jpg

Cat No. GTX31540

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

Human Liver Tissue Slide , Human Liver Tissue Lysate
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RNF20 antibody was raised against a 19 amino acid peptide near the amino terminus of human RNF20.The immunogen is located within amino acids 130 - 180 of RNF20.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ring Finger Protein 20,Bre1,Bre1A,Hbre1,Rnf20

Background

The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]

Database

DATA IMAGES
GTX31540_1150_IHC-P_20180221_18121410_729.jpg

GTX31540 IHC-P Image

IHC-P analysis of human liver tissue using GTX31540 RNF20 antibody.
Working concentration : 2.5 μg/ml

GTX31540_2725_WB_20180221_18121410_249.jpg

GTX31540 WB Image

WB analysis of human liver tissue lysate using GTX31540 RNF20 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

GTX31540_1151_IHC-P_20180221_18121410_801.jpg

GTX31540 IHC-P Image

IHC-P analysis of human liver tissue using GTX31540 RNF20 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329