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RNF23 antibody [N2C2], Internal

Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637
Anti-RNF23 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116637
Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637
Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637

Cat No. GTX116637

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all RNF23 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A431 , mouse testis , rat testis

Predict Reactivity

Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNF23. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618782

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 39 , RNF23 , TFP , TRIM39B

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. This gene lies within the major histocompatibility complex class I region on chromosome 6. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637

GTX116637 WB Image

Sample (50 ug of whole cell lysate)
A: Rat testis
7.5% SDS PAGE
GTX116637 diluted at 1:1000

Anti-RNF23 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116637

GTX116637 IHC-P Image

RNF23 antibody [N2C2], Internal detects TRIM39 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
RNF23 antibody [N2C2], Internal (GTX116637) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637

GTX116637 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX116637 diluted at 1:1000

Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637

GTX116637 WB Image

Sample (50 ug of whole cell lysate)
A: mouse testis
7.5% SDS PAGE
GTX116637 diluted at 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169