GeneTex
United States (US)

RNF25 antibody [C2C3], C-term

GTX104668_39638_IFA_19011506_436.jpg
GTX104668_39638_IHC_19011506_169.jpg
GTX104668_WB_19011506_441.jpg

Cat No. GTX104668

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

H1299
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RNF25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ring Finger Protein 25,Ao7,Rnf25

Background

The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq]

Database

DATA IMAGES
GTX104668_39638_IFA_19011506_436.jpg

GTX104668 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using RNF25(GTX104668) antibody at 1:200 dilution.

GTX104668_39638_IHC_19011506_169.jpg

GTX104668 IHC-P Image

RNF25 antibody [C2C3], C-term detects RNF25 protein at nucleus and cytosol on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
RNF25 antibody [C2C3], C-term (GTX104668) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104668_WB_19011506_441.jpg

GTX104668 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX104668 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169