APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 4-8μg/ml |
|
Note :
IHC-P
Human Kidney shows cytoplasm staining in select DCT.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89815-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_919247.1 and NP_079402.2).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence KAGATSMWASCC, from the N Terminus of the protein sequence according to NP_919247.1; NP_079402.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ring finger protein 34 , CARP-1 , CARP1 , RFI , RIF , RIFF , hRFI
Cellular Localization
Cell membrane , Nucleus , Nucleus speckle , Cytoplasm, cytosol
Background
The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
Database
Research Area
DATA IMAGES
|
GTX89815 IHC-P Image
IHC-P analysis of human kidney using GTX89815 RNF34 antibody, N-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 4μg/ml
|
REFERENCE
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REVIEW
There are currently no reviews for RNF34 antibody, N-term (GTX89815). Be the first to share your experience with this product.
