APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2μg/ml |
| 2.5-3.8μg/ml |
|
Note :
IHC-P
Human Testis shows speckled staining in nuclei of spermatocytes and spermitids.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89832-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).
Predict Reactivity
Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GENKEPLHILHPQ, from the C Terminus of the protein sequence according to NP_892030.3; NP_542783.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tripartite motif containing 69 , HSD-34 , HSD34 , RNF36 , Trif
Cellular Localization
Cytoplasm , Nucleus , Nucleus speckle
Background
This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89832 WB Image
WB analysis of mouse testis lysate using GTX89832 RNF36 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
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GTX89832 IHC-P Image
IHC-P analysis of human testis using GTX89832 RNF36 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for RNF36 antibody, C-term (GTX89832). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RNF36 antibody, C-term (GTX89832). Be the first to share your experience with this product.
