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RNF40 antibody [N1N2], N-term

KO/KD Orthogonal
Anti-RNF40 antibody [N1N2], N-term used in Western Blot (WB). GTX115295
Anti-RNF40 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115295
Anti-RNF40 antibody [N1N2], N-term used in Western Blot (WB). GTX115295
Anti-RNF40 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115295
Anti-RNF40 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX115295

Cat. No. GTX115295

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RNF40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

IMR32 , HeLa , HeLa NE

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RNF40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176555

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 40 , BRE1B , RBP95 , STARING

Cellular Localization

Nucleus

Background

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RNF40 antibody [N1N2], N-term used in Western Blot (WB). GTX115295

GTX115295 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115295 diluted at 1:1000

Anti-RNF40 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115295

GTX115295 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RNF40(GTX115295) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RNF40 antibody [N1N2], N-term used in Western Blot (WB). GTX115295

GTX115295 WB Image

Orthogonal

RNF40 antibody detects RNF40 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNF40 antibody (GTX115295) diluted by 1:1000.

Anti-RNF40 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115295

GTX115295 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using RNF40(GTX115295) antibody at 1:500 dilution.

Anti-RNF40 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX115295

GTX115295 IP Image

Immunoprecipitation of RNF40 protein from HeLa whole cell extracts using 5 μg of RNF40 antibody [N1N2], N-term (GTX115295).
Western blot analysis was performed using RNF40 antibody [N1N2], N-term (GTX115295).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Fujimoto M et al. Nat Commun 2022; 13 (1) : 4355 HSF1 phosphorylation establishes an active chromatin state via the TRRAP-TIP60 complex and promotes tumorigenesis.
Application : WB
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169