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RNF41 antibody

Anti-RNF41 antibody used in Western Blot (WB). GTX115366
Anti-RNF41 antibody used in IHC (Paraffin sections) (IHC-P). GTX115366
Anti-RNF41 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115366
Anti-RNF41 antibody used in IHC (Paraffin sections) (IHC-P). GTX115366
Anti-RNF41 antibody used in Western Blot (WB). GTX115366

Cat. No. GTX115366

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RNF41 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNF41. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620408

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 41 , FLRF , NRDP1 , SBBI03

Background

The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. The specific function of this protein has not yet been determined. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RNF41 antibody used in Western Blot (WB). GTX115366

GTX115366 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115366 diluted at 1:3000

Anti-RNF41 antibody used in IHC (Paraffin sections) (IHC-P). GTX115366

GTX115366 IHC-P Image

RNF41 antibody detects RNF41 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
RNF41 antibody (GTX115366) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RNF41 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115366

GTX115366 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using RNF41(GTX115366) antibody at 1:500 dilution.

Anti-RNF41 antibody used in IHC (Paraffin sections) (IHC-P). GTX115366

GTX115366 IHC-P Image

The data was published in the journal Theranostics in 2019. PMID: 31754404

Anti-RNF41 antibody used in Western Blot (WB). GTX115366

GTX115366 WB Image

The data was published in the journal Theranostics in 2019. PMID: 31754404

REFERENCE

Application Reference

Lian YF et al. Theranostics 2019; 9 (26) : 8392-8408 CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169