GeneTex
United States (US)

RNF8 antibody, N-term

RNF8-antibody-N-term-GTX15850-WB-1_18121411_207.jpg

Cat No. GTX15850

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-1μg/ml
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX15850-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence GEPGFFVTGDRAG-C, from the N Terminus of the protein sequence according to NP_003949.1; NP_898901.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ring Finger Protein 8,Hrnf8,Rnf8

Cellular Localization

Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pa

Background

The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES
RNF8-antibody-N-term-GTX15850-WB-1_18121411_207.jpg

GTX15850 WB Image

GTX15850 staining (0.1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Package List Price ($)
$ 329