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RNase H1 antibody [HL2343]

Recombinant antibody
Anti-RNase H1 antibody [HL2343] used in Western Blot (WB). GTX638546
Anti-RNase H1 antibody [HL2343] used in IHC (Paraffin sections) (IHC-P). GTX638546
Anti-RNase H1 antibody [HL2343] used in Western Blot (WB). GTX638546
Anti-RNase H1 antibody [HL2343] used in Western Blot (WB). GTX638546
Anti-RNase H1 antibody [HL2343] used in IHC (Paraffin sections) (IHC-P). GTX638546

Cat. No. GTX638546

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2343

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Dog
Package
100 μl ($399),
25 μl ($169)
See all RNase H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human RNase H1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonuclease H1 , H1RNA , PEOB2 , RNH1

Cellular Localization

Cytoplasm

Background

This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-RNase H1 antibody [HL2343] used in Western Blot (WB). GTX638546

GTX638546 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [HL2343] (GTX638546) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RNase H1 antibody [HL2343] used in IHC (Paraffin sections) (IHC-P). GTX638546

GTX638546 IHC-P Image

RNase H1 antibody [HL2343] detects RNase H1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RNase H1 stained by RNase H1 antibody [HL2343] (GTX638546) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RNase H1 antibody [HL2343] used in Western Blot (WB). GTX638546

GTX638546 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [HL2343] (GTX638546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RNase H1 antibody [HL2343] used in Western Blot (WB). GTX638546

GTX638546 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [HL2343] (GTX638546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RNase H1 antibody [HL2343] used in IHC (Paraffin sections) (IHC-P). GTX638546

GTX638546 IHC-P Image

RNase H1 antibody [HL2343] detects RNase H1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog tissues.
RNase H1 stained by RNase H1 antibody [HL2343] (GTX638546) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations