RNase H1 antibody [N2C3]

Cat. No. GTX117624

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all RNase H1 products

View product citations for antibody GTX117624 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa , whole zebrafish

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNase H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729209

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonuclease H1 , H1RNA , PEOB2 , RNH1

Cellular Localization

Cytoplasm

Database

Gene ID: 246243 RNASEH1
UniProt: O60930 RNASEH1

Research Area

DATA IMAGES

GTX117624 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [N2C3] (GTX117624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX117624 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [N2C3] (GTX117624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX117624 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RNase H1(GTX117624) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min