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RNase L antibody [HL2340]

Recombinant antibody
Anti-RNase L antibody [HL2340] used in Western Blot (WB). GTX638543
Anti-RNase L antibody [HL2340] used in IHC (Paraffin sections) (IHC-P). GTX638543
Anti-RNase L antibody [HL2340] used in IHC (Paraffin sections) (IHC-P). GTX638543
Anti-RNase L antibody [HL2340] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638543

Cat. No. GTX638543

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2340

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RNase L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human RNase L

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonuclease L , PRCA1 , RNS4

Cellular Localization

Cytoplasm,Mitochondrion

Background

This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RNase L antibody [HL2340] used in Western Blot (WB). GTX638543

GTX638543 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2340] (GTX638543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RNase L antibody [HL2340] used in IHC (Paraffin sections) (IHC-P). GTX638543

GTX638543 IHC-P Image

RNase L antibody [HL2340] detects RNase L protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RNase L stained by RNase L antibody [HL2340] (GTX638543) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RNase L antibody [HL2340] used in IHC (Paraffin sections) (IHC-P). GTX638543

GTX638543 IHC-P Image

RNase L antibody [HL2340] detects RNase L protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RNase L stained by RNase L antibody [HL2340] (GTX638543) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RNase L antibody [HL2340] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638543

GTX638543 ICC/IF Image

RNase L antibody [HL2340] detects RNase L protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RNase L stained by RNase L antibody [HL2340] (GTX638543) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations