ROBO1 antibody [C2C3], C-term

Cat No. GTX113826

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μl ($329), 25 μl ($169)

See all ROBO1 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:5000-1:20000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Positive Control

HeLa , A375

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ROBO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885333

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

roundabout guidance receptor 1 , DUTT1 , SAX3

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 6091 ROBO1
UniProt: Q9Y6N7 ROBO1

Research Area

DATA IMAGES

GTX113826 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [C2C3], C-term (GTX113826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX113826 IHC-P Image

ROBO1 antibody [C2C3], C-term detects ROBO1 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
ROBO1 antibody [C2C3], C-term (GTX113826) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 329

25 μl

$ 169

Qty

ROBO1 antibody [C2C3], C-term

Cat No. GTX113826

Host

Clonality

Isotype

Application

Reactivity