APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:5000-1:20000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , A375
Predict Reactivity
Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human ROBO1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885333
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
roundabout guidance receptor 1 , DUTT1 , SAX3
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX113826 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [C2C3], C-term (GTX113826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
GTX113826 IHC-P Image
ROBO1 antibody [C2C3], C-term detects ROBO1 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. ROBO1 antibody [C2C3], C-term (GTX113826) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
REFERENCE
There are currently no references for ROBO1 antibody [C2C3], C-term (GTX113826). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ROBO1 antibody [C2C3], C-term (GTX113826). Be the first to share your experience with this product.