GeneTex
United States (US)

ROBO1 antibody [C2C3], C-term

GTX113826_41066_WB_18121410_777.jpg
GTX113826_41066_IHC_18121410_952.jpg

Cat No. GTX113826

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Positive Control

HeLa , A375

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ROBO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DUTT1 antibody, FLJ21882 antibody, MGC131599 antibody, MGC133277 antibody, SAX3 antibody, ROBO1 antibody, roundabout homolog 1 antibody, deleted in U twenty twenty antibody, roundabout, axon guidance receptor, homolog 1 (Drosophila) antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX113826_41066_WB_18121410_777.jpg

GTX113826 WB Image

ROBO1 antibody [C2C3], C-term detects ROBO1 protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg A375 whole cell lysate/extract
5% SDS PAGE
ROBO1 antibody [C2C3], C-term (GTX113826) dilution: 1:10000

GTX113826_41066_IHC_18121410_952.jpg

GTX113826 IHC-P Image

ROBO1 antibody [C2C3], C-term detects ROBO1 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
ROBO1 antibody [C2C3], C-term (GTX113826) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf