*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLa , A375
Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)
Recombinant protein encompassing a sequence within the C-terminus region of human ROBO1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DUTT1 antibody, FLJ21882 antibody, MGC131599 antibody, MGC133277 antibody, SAX3 antibody, ROBO1 antibody, roundabout homolog 1 antibody, deleted in U twenty twenty antibody, roundabout, axon guidance receptor, homolog 1 (Drosophila) antibody
Membrane; Single-pass type I membrane protein
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX113826 WB Image
ROBO1 antibody [C2C3], C-term detects ROBO1 protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg A375 whole cell lysate/extract
5% SDS PAGE
ROBO1 antibody [C2C3], C-term (GTX113826) dilution: 1:10000
GTX113826 IHC-P Image
ROBO1 antibody [C2C3], C-term detects ROBO1 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
ROBO1 antibody [C2C3], C-term (GTX113826) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min