GeneTex

  • Country / Location Selection

United States (US)

ROBO1 antibody [HL2559]

Recombinant antibody Orthogonal
Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936
Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936
Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936
Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936

Cat. No. GTX638936

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2559

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ROBO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Product Note

This antibody was raised against human ROBO1 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ROBO1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

roundabout guidance receptor 1 , DUTT1 , SAX3

Cellular Localization

Cell membrane,Cell projection, axon

Background

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936

GTX638936 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [HL2559] (GTX638936) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936

GTX638936 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [HL2559] (GTX638936) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936

GTX638936 IHC-P Image

ROBO1 antibody [HL2559] detects ROBO1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
ROBO1 stained by ROBO1 antibody [HL2559] (GTX638936) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936

GTX638936 IHC-P Image

ROBO1 antibody [HL2559] detects ROBO1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ROBO1 stained by ROBO1 antibody [HL2559] (GTX638936) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for ROBO1 antibody [HL2559] (GTX638936). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ROBO1 antibody [HL2559] (GTX638936). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations