APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 2μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89308-PEP).
Product Note
This antibody is expected to recognise all reported isoforms (NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
roundabout guidance receptor 1 , DUTT1 , SAX3
Cellular Localization
Cell membrane
Background
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Database
Research Area
DATA IMAGES
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GTX89308 WB Image
WB analysis of HUVEC lysate using GTX89308 ROBO1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89308 IHC-P Image
IHC-P analysis of human liver using GTX89308 ROBO1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
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REFERENCE
There are currently no references for ROBO1 antibody, Internal (GTX89308). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ROBO1 antibody, Internal (GTX89308). Be the first to share your experience with this product.
