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ROBO1 antibody

Anti-ROBO1 antibody used in Western Blot (WB). GTX36928
Anti-ROBO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX36928
Anti-ROBO1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX36928
Anti-ROBO1 antibody used in Western Blot (WB). GTX36928
Anti-ROBO1 antibody used in ELISA (ELISA). GTX36928
Anti-ROBO1 antibody used in Western Blot (WB). GTX36928
Anti-ROBO1 antibody used in Western Blot (WB). GTX36928
Anti-ROBO1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX36928

Cat. No. GTX36928

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($589)
See all ROBO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 2-10 μg/mL
IHC-Fr 2-10 μg/mL
IP Assay dependent
ELISA 1:30000-1:60000
Not tested in other applications.

Calculated MW

181 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

roundabout guidance receptor 1 , DUTT1 , SAX3

Cellular Localization

Cell membrane

Background

ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2. The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein. ROBO-1 is a widely expressed protein with the exception of the kidney. Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.

Database

Research Area

DATA IMAGES

Anti-ROBO1 antibody used in Western Blot (WB). GTX36928

GTX36928 WB Image

Western blot using Affinity Purified anti-ROBO-1 antibody (GTX36928) shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 μg of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of infrared 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Infrared 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.

Anti-ROBO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX36928

GTX36928 IHC-P Image

Affinity Purified anti-ROBO1 antibody (GTX36928) was used at a concentration of 5 μg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.

Anti-ROBO1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX36928

GTX36928 IHC-Fr Image

1/50 staining mouse lung tissue sections (adult, frozen 100μm wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with polyethylene glycol tert-octylphenyl ether before incubation with the antibody (GTX36928) for 16 hours at 4ºC.

Anti-ROBO1 antibody used in Western Blot (WB). GTX36928

GTX36928 WB Image

WB analysis of mouse brain tissue lysate using GTX36928 ROBO1 antibody.
Loading : 35 μg
Dilution : 1:1000

Anti-ROBO1 antibody used in ELISA (ELISA). GTX36928

GTX36928 ELISA Image

ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX36928 ROBO1 antibody.
Coating : 0.1 μg

Anti-ROBO1 antibody used in Western Blot (WB). GTX36928

GTX36928 WB Image

WB analysis of various samples using GTX36928 ROBO1 antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : LNCap whole cell lysate
Lane 8 : Molt-4 whole cell lysate
Lane 9 : Ramos whole cell lysate
Lane 10 : Raji whole cell lysate
Lane 11 : A-172 whole cell lysate
Lane 12 : NIH-3T3 whole cell lysate
Loading : 35 μg
Dilution : 1 μg/mL

Anti-ROBO1 antibody used in Western Blot (WB). GTX36928

GTX36928 WB Image

WB analysis of HeLa whole cell lysate using GTX36928 ROBO1 antibody.
Loading : 30 μg
Dilution : 1:1000

Anti-ROBO1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX36928

GTX36928 IHC-Fr Image

IHC-Fr analysis of mouse lung tissue using GTX36928 ROBO1 antibody.
Permeabilization : Triton X-100
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589