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ROCK1 + ROCK2 antibody [HL1632]

Recombinant antibody Overexpression
Anti-ROCK1 + ROCK2 antibody [HL1632] used in Western Blot (WB). GTX637125
Anti-ROCK1 + ROCK2 antibody [HL1632] used in IHC (Paraffin sections) (IHC-P). GTX637125
Anti-ROCK1 + ROCK2 antibody [HL1632] used in Western Blot (WB). GTX637125
Anti-ROCK1 + ROCK2 antibody [HL1632] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637125
Anti-ROCK1 + ROCK2 antibody [HL1632] used in Western Blot (WB). GTX637125
Anti-ROCK1 + ROCK2 antibody [HL1632] used in Western Blot (WB). GTX637125
Anti-ROCK1 + ROCK2 antibody [HL1632] used in IHC (Paraffin sections) (IHC-P). GTX637125
Anti-ROCK1 + ROCK2 antibody [HL1632] used in IHC (Paraffin sections) (IHC-P). GTX637125

Cat. No. GTX637125

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1632

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ROCK1 + ROCK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human ROCK1 .

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 1 , Rho associated coiled-coil containing protein kinase 2 , P160ROCK , ROCK-I , ROCK-II

Cellular Localization

Cytoplasm,Cell membrane,Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm, cytoskeleton,Cell projection, lamellipodium

Database

DATA IMAGES

Anti-ROCK1 + ROCK2 antibody [HL1632] used in Western Blot (WB). GTX637125

GTX637125 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 + ROCK2 antibody [HL1632] used in IHC (Paraffin sections) (IHC-P). GTX637125

GTX637125 IHC-P Image

ROCK1 + ROCK2 antibody [HL1632] detects ROCK1 + ROCK2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ROCK1 + ROCK2 stained by ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK1 + ROCK2 antibody [HL1632] used in Western Blot (WB). GTX637125

GTX637125 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 + ROCK2 antibody [HL1632] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637125

GTX637125 ICC/IF Image

ROCK1 + ROCK2 antibody [HL1632] detects ROCK1 + ROCK2 protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROCK1 + ROCK2 stained by ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ROCK1 + ROCK2 antibody [HL1632] used in Western Blot (WB). GTX637125

GTX637125 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 + ROCK2 antibody [HL1632] used in Western Blot (WB). GTX637125

GTX637125 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 + ROCK2 antibody [HL1632] used in IHC (Paraffin sections) (IHC-P). GTX637125

GTX637125 IHC-P Image

ROCK1 + ROCK2 antibody [HL1632] detects ROCK1 + ROCK2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
ROCK1 + ROCK2 stained by ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK1 + ROCK2 antibody [HL1632] used in IHC (Paraffin sections) (IHC-P). GTX637125

GTX637125 IHC-P Image

ROCK1 + ROCK2 antibody [HL1632] detects ROCK1 + ROCK2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ROCK1 + ROCK2 stained by ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations