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ROCK1 antibody [1H4]

Anti-ROCK1 antibody [1H4] used in Western Blot (WB). GTX60535
Anti-ROCK1 antibody [1H4] used in Western Blot (WB). GTX60535
Anti-ROCK1 antibody [1H4] used in ELISA (ELISA). GTX60535

Cat No. GTX60535

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H4

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
100 μg ($379)
See all ROCK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ELISA 1/10000
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ROCK1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ROCK1 antibody [1H4] used in Western Blot (WB). GTX60535

GTX60535 WB Image

WB analysis of HEK293 (1) and ROCK1 (AA: 403-610)-hIgGFc transfected HEK293 (2) cell lysate using GTX60535 ROCK1 antibody [1H4].

Anti-ROCK1 antibody [1H4] used in Western Blot (WB). GTX60535

GTX60535 WB Image

WB analysis of human ROCK1 (AA: 403-610) recombinant protein using GTX60535 ROCK1 antibody [1H4].

Anti-ROCK1 antibody [1H4] used in ELISA (ELISA). GTX60535

GTX60535 ELISA Image

ELISA analysis of antigen using GTX60535 ROCK1 antibody [1H4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379