APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human ROCK1 expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane
Background
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX60535 WB Image
WB analysis of HEK293 (1) and ROCK1 (AA: 403-610)-hIgGFc transfected HEK293 (2) cell lysate using GTX60535 ROCK1 antibody [1H4].
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GTX60535 WB Image
WB analysis of human ROCK1 (AA: 403-610) recombinant protein using GTX60535 ROCK1 antibody [1H4].
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GTX60535 ELISA Image
ELISA analysis of antigen using GTX60535 ROCK1 antibody [1H4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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REFERENCE
There are currently no references for ROCK1 antibody [1H4] (GTX60535). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ROCK1 antibody [1H4] (GTX60535). Be the first to share your experience with this product.