APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , HeLa , HepG2 , Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , PC-12 (30uM cisplatin for 24hr)
Predict Reactivity
Zebrafish, Rabbit, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888179
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
Cellular Localization
Cytoplasm , Centrosome , centriole , Golgi apparatus membrane; Peripheral membrane protein
Background
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/templates/default/js/lightbox/images/loading.gif "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
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GTX629971 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT261] (GTX629971) diluted at 1:2000.
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GTX629971 WB Image
Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT261] (GTX629971) diluted at 1:500.
The observed M.W. is based on the publication: PMID: 23392171, PMID: 16983089
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GTX629971 WB Image
ROCK1 antibody [GT261] detects ROCK1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
ROCK1 antibody [GT261] (GTX629971) dilution: 1:1000
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GTX629971 WB Image
ROCK1 antibody [GT261] detects ROCK1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
ROCK1 antibody [GT261] (GTX629971) dilution: 1:1000
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GTX629971 WB Image
ROCK1 antibody [GT261] detects ROCK1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
ROCK1 antibody [GT261] (GTX629971) dilution: 1:1000
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REFERENCE
There are currently no references for ROCK1 antibody [GT261] (GTX629971). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ROCK1 antibody [GT261] (GTX629971). Be the first to share your experience with this product.
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/small/GTX629971/GTX629971_41484_20160728_WB_shRNA_watermark_18121411_355.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/small/GTX629971/GTX629971_41484_20160616_WB_Cisplatin_18121411_987.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/small/GTX629971/GTX629971_41484_WB_18121411_154.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/small/GTX629971/GTX629971_41484_WB_R_18121411_381.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/small/GTX629971/GTX629971_41484_WB_M_18121411_481.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/normal/GTX629971/GTX629971_41484_20160728_WB_shRNA_watermark_18121411_355.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/normal/GTX629971/GTX629971_41484_20160616_WB_Cisplatin_18121411_987.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/normal/GTX629971/GTX629971_41484_WB_18121411_154.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/normal/GTX629971/GTX629971_41484_WB_R_18121411_381.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")
![Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971](/upload/website/prouct_img/normal/GTX629971/GTX629971_41484_WB_M_18121411_481.jpg "Anti-ROCK1 antibody [GT261] used in Western Blot (WB). GTX629971")