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ROCK1 antibody [GT464]

Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972
Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972
Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972
Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972
Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972
Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972

Cat No. GTX629972

Host

Mouse

Clonality

Monoclonal

Clone Name

GT464

Isotype

IgG2b

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , PC-12 (30uM cisplatin for 24hr)

Predict Reactivity

Zebrafish, Rabbit, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888180

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I

Cellular Localization

Cytoplasm , Centrosome , centriole , Golgi apparatus membrane; Peripheral membrane protein

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972

GTX629972 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT464] (GTX629972) diluted at 1:3000.

Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972

GTX629972 WB Image

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT464] (GTX629972) diluted at 1:500.
The observed M.W. is based on the publication: PMID: 23392171, PMID: 16983089

Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972

GTX629972 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK1 antibody [GT464] (GTX629972) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972

GTX629972 WB Image

ROCK1 antibody [GT464] detects ROCK1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
ROCK1 antibody [GT464] (GTX629972) dilution: 1:1000

Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972

GTX629972 WB Image

ROCK1 antibody [GT464] detects ROCK1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
ROCK1 antibody [GT464] (GTX629972) dilution: 1:1000

Anti-ROCK1 antibody [GT464] used in Western Blot (WB). GTX629972

GTX629972 WB Image

ROCK1 antibody [GT464] detects ROCK1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
ROCK1 antibody [GT464] (GTX629972) dilution: 1:1000

REFERENCE

There are currently no references for ROCK1 antibody [GT464] (GTX629972). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ROCK1 antibody [GT464] (GTX629972). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations