APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , NIH-3T3 , C2Cl2 , rat brain
Predict Reactivity
Zebrafish, Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.89 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037902
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
Cellular Localization
Cytoplasm , Centrosome , centriole , Golgi apparatus membrane; Peripheral membrane protein
Background
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113266 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113266 WB Image
Wild-type (WT) and ROCK1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113266 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX113266 ICC/IF Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ROCK1 protein stained by ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX113266 IHC-P Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113266 WB Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. A. 50 μg rat brain lysate/extract 5% SDS-PAGE ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113266 WB Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. A. 30 μg NIH-3T3 whole cell extract B. 30 μg C2Cl2 whole cell extract 5% SDS-PAGE ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113266 IP Image
ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 μg of ROCK1 antibody (GTX113266) 5% SDS-PAGE The immunoprecipitated ROCK1 protein was detected by ROCK1 antibody (GTX113266) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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GTX113266 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113266 ICC/IF Image
ROCK1 antibody [N1N2], N-term detects ROCK1 protein at nucleus and Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ROCK1 stained by ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
REVIEW
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