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ROCK1 antibody [N1N2], N-term

KO/KD Comparison
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266
Anti-ROCK1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113266

Cat No. GTX113266

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all ROCK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , C2Cl2 , rat brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037902

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I

Cellular Localization

Cytoplasm , Centrosome , centriole , Golgi apparatus membrane; Peripheral membrane protein

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266

GTX113266 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266

GTX113266 WB Image

KO/KD

Wild-type (WT) and ROCK1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266

GTX113266 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ROCK1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113266

GTX113266 ICC/IF Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROCK1 protein stained by ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ROCK1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113266

GTX113266 IHC-P Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266

GTX113266 WB Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
5% SDS-PAGE
ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266

GTX113266 WB Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell extract
B. 30 μg C2Cl2 whole cell extract
5% SDS-PAGE
ROCK1 antibody [N1N2], N-term (GTX113266) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX113266

GTX113266 IP Image

ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 μg of ROCK1 antibody (GTX113266) 5% SDS-PAGE The immunoprecipitated ROCK1 protein was detected by ROCK1 antibody (GTX113266) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-ROCK1 antibody [N1N2], N-term used in Western Blot (WB). GTX113266

GTX113266 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113266

GTX113266 ICC/IF Image

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at nucleus and Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROCK1 stained by ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Yamada S et al. J Tissue Eng 2021; 12 : 20417314211019300 Induction of osteogenic differentiation of bone marrow stromal cells on 3D polyester-based scaffolds solely by subphysiological fluidic stimulation in a laminar flow bioreactor.
Application : ICC/IF
Reactivity : Rat
Steurer S et al. Aging (Albany NY) 2019; 11 (18) : 7859-7879 Up regulation of Rho-associated coiled-coil containing kinase1 (ROCK1) is associated with genetic instability and poor prognosis in prostate cancer.
Jung HJ et al. Anticancer Res 2019; 39 (11) : 6165-6173 Effect of Tissue Transglutaminase on Steroid Resistance in T-Cell Acute Lymphoblastic Leukemia.
Application : WB
Reactivity : Human
Yahiro K et al. Cell Microbiol 2019; : e13033 Cholix toxin, an eukaryotic elongation factor 2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes.
Application : WB
Reactivity : Human
Tsuchiya M et al. Nat Commun 2018; 9 (1) : 2049 Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation.
Application : ICC/IF
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169