GeneTex
United States (US)

ROCK1 antibody

GTX125921_40842_20160728_WB_shRNA_watermark_18121410_460.jpg
GTX125921_40842_WB_18121410_528.jpg

Cat No. GTX125921

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ROCK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho Associated Coiled-Coil Containing Protein Kinase 1,P160Rock,Rock-I,Rock1

Cellular Localization

Cytoplasm , Centrosome , centriole , Golgi apparatus membrane; Peripheral membrane protein

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX125921_40842_20160728_WB_shRNA_watermark_18121410_460.jpg

GTX125921 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody (GTX125921) diluted at 1:500.

GTX125921_40842_WB_18121410_528.jpg

GTX125921 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX125921 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169