Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody (GTX125921) diluted at 1:500.

Sample (30 ug of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX125921 diluted at 1:500