APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 10 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)
Product Note
ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1.The immunogen is located within the first 50 amino acids of ROCK1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane
Background
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31836 ICC/IF Image
ICC/IF analysis of 293 cells using GTX31836 ROCK1 antibody.
Working concentration : 20 μg/ml
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GTX31836 ICC/IF Image
ICC/IF analysis of 293 cells using GTX31836 ROCK1 antibody.
Working concentration : 10 μg/ml
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GTX31836 WB Image
WB analysis of 293 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31836 ROCK1 antibody.
Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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