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ROCK1 antibody

Anti-ROCK1 antibody used in Western Blot (WB). GTX31836
Anti-ROCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31836
Anti-ROCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31836

Cat No. GTX31836

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

Specificity/Sensitivity

ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1.The immunogen is located within the first 50 amino acids of ROCK1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ROCK1 antibody used in Western Blot (WB). GTX31836

GTX31836 WB Image

WB analysis of 293 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31836 ROCK1 antibody.
Working concentration : 1 μg/ml

Anti-ROCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31836

GTX31836 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31836 ROCK1 antibody.
Working concentration : 10 μg/ml

Anti-ROCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31836

GTX31836 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31836 ROCK1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339