APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:100 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane
Background
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]
Database
Research Area
DATA IMAGES
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GTX32843 IP Image
IP analysis of 293T cell lysate using GTX32843 ROCK1 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000
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GTX32843 WB Image
WB analysis of various sample lysates using GTX32843 ROCK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX32843 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX32843 ROCK1 antibody.
Dilution : 1:100
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GTX32843 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32843 ROCK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
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REVIEW
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