GeneTex
United States (US)

ROCK1 antibody

KO/KD
GTX32843_20180530_IHC-P_18121410_113.jpg
GTX32843_20180530_WB_18121410_514.jpg
GTX32843_20180822_WB_18121410_427.jpg
GTX32843_20180530_IHC-P_1_18121410_511.jpg

Cat No. GTX32843

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

158 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho Associated Coiled-Coil Containing Protein Kinase 1,P160Rock,Rock-I,Rock1

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32843_20180530_IHC-P_18121410_113.jpg

GTX32843 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32843 ROCK1 antibody.
Dilution : 1:100

GTX32843_20180530_WB_18121410_514.jpg

GTX32843 WB Image

WB analysis of various samples using GTX32843 ROCK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32843_20180822_WB_18121410_427.jpg

GTX32843 WB Image

KO/KD

WB analysis of wild type and ROCK1 knockout cell lysate using GTX32843 ROCK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32843_20180530_IHC-P_1_18121410_511.jpg

GTX32843 IHC-P Image

IHC-P analysis of rat heart tissue using GTX32843 ROCK1 antibody.
Dilution : 1:100

Package List Price ($)
$ 329