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ROCK1 antibody

Anti-ROCK1 antibody used in Western Blot (WB). GTX32843
Anti-ROCK1 antibody used in Western Blot (WB). GTX32843
Anti-ROCK1 antibody used in Immunoprecipitation (IP). GTX32843
Anti-ROCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32843

Cat No. GTX32843

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:100
IP 1:50-1:100
Not tested in other applications.

Calculated MW

158 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-ROCK1 antibody used in Western Blot (WB). GTX32843

GTX32843 WB Image

WB analysis of normal (control) and ROCK1 knockout (KO) HeLa cell lysate using GTX32843 ROCK1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-ROCK1 antibody used in Western Blot (WB). GTX32843

GTX32843 WB Image

WB analysis of various sample lysates using GTX32843 ROCK1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-ROCK1 antibody used in Immunoprecipitation (IP). GTX32843

GTX32843 IP Image

IP analysis of 293T cells using GTX32843 ROCK1 antibody.
IP reaction : 200μg extracts / 3μg antibody antibody
Dilution : 1:1000

Anti-ROCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32843

GTX32843 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX32843 ROCK1 antibody.
Dilution : 1:100

REFERENCE

There are currently no references for ROCK1 antibody (GTX32843). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339