APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , 293T , A431 , HeLa , HepG2
Product Note
This antibody is specific for human ROCK2 protein, and it does not cross react with human ROCK1 protein.
Predict Reactivity
Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888338
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho associated coiled-coil containing protein kinase 2 , ROCK-II
Cellular Localization
Cytoplasm , Cell membrane; Peripheral membrane protein
Background
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/templates/default/js/lightbox/images/loading.gif "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")
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GTX633389 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [GT11712] (GTX633389) diluted at 1:500.
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GTX633389 IHC-P Image
ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ROCK2 antibody [GT11712] (GTX633389) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633389 IHC-P Image
ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in human esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
ROCK2 antibody [GT11712] (GTX633389) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633389 IHC-P Image
ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ROCK2 antibody [GT11712] (GTX633389) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633389 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [GT11712] (GTX633389) diluted at 1:1000 and competitor's antibody (sc-1851) diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX633389 IHC-P Image
ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ROCK2 antibody [GT11712] (GTX633389) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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|
GTX633389 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [GT11712] (GTX633389) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX633389 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [GT11712] (GTX633389) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for ROCK2 antibody [GT11712] (GTX633389). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ROCK2 antibody [GT11712] (GTX633389). Be the first to share your experience with this product.
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/upload/website/prouct_img/small/GTX633389/GTX633389_42506_20160901_WB_shRNA_watermark_w_23061202_895.webp "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389](/upload/website/prouct_img/small/GTX633389/GTX633389_42506_20160713_IHC-P_M_w_23061202_618.webp "Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389](/upload/website/prouct_img/small/GTX633389/GTX633389_42506_20160713_IHC-P_H_w_23061202_865.webp "Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389](/upload/website/prouct_img/small/GTX633389/GTX633389_42506_20160713_IHC-P_R_w_23061202_378.webp "Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/upload/website/prouct_img/small/GTX633389/GTX633389_42506_20171222_WB_competitor_watermark_w_23061202_452.webp "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389](/upload/website/prouct_img/small/GTX633389/GTX633389_42506_20160713_IHC-P_M_2_w_23061202_170.webp "Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/upload/website/prouct_img/small/GTX633389/GTX633389_44881_20221202_WB_TPM_watermark_22122722_841.webp "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/upload/website/prouct_img/small/GTX633389/GTX633389_44881_20230818_WB_multiple_B_23082201_988.webp "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/upload/website/prouct_img/normal/GTX633389/GTX633389_42506_20160901_WB_shRNA_watermark_w_23061202_895.webp "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389](/upload/website/prouct_img/normal/GTX633389/GTX633389_42506_20160713_IHC-P_M_w_23061202_618.webp "Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389](/upload/website/prouct_img/normal/GTX633389/GTX633389_42506_20160713_IHC-P_H_w_23061202_865.webp "Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389](/upload/website/prouct_img/normal/GTX633389/GTX633389_42506_20160713_IHC-P_R_w_23061202_378.webp "Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/upload/website/prouct_img/normal/GTX633389/GTX633389_42506_20171222_WB_competitor_watermark_w_23061202_452.webp "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389](/upload/website/prouct_img/normal/GTX633389/GTX633389_42506_20160713_IHC-P_M_2_w_23061202_170.webp "Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/upload/website/prouct_img/normal/GTX633389/GTX633389_44881_20221202_WB_TPM_watermark_22122722_841.webp "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")
![Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389](/upload/website/prouct_img/normal/GTX633389/GTX633389_44881_20230818_WB_multiple_B_23082201_988.webp "Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389")