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ROCK2 antibody [GT11712]

Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389
Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389
Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389
Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389
Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389
Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389
Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389

Cat No. GTX633389

Host

Mouse

Clonality

Monoclonal

Clone Name

GT11712

Isotype

IgG1

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549

Predict Reactivity

Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

4.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888338

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389

GTX633389 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [GT11712] (GTX633389) diluted at 1:500.

Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389

GTX633389 IHC-P Image

ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ROCK2 antibody [GT11712] (GTX633389) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389

GTX633389 IHC-P Image

ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in human esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
ROCK2 antibody [GT11712] (GTX633389) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389

GTX633389 IHC-P Image

ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ROCK2 antibody [GT11712] (GTX633389) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 antibody [GT11712] used in IHC (Paraffin sections) (IHC-P). GTX633389

GTX633389 IHC-P Image

ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ROCK2 antibody [GT11712] (GTX633389) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389

GTX633389 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX633389) diluted at 1:1000.

Anti-ROCK2 antibody [GT11712] used in Western Blot (WB). GTX633389

GTX633389 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [GT11712] (GTX633389) diluted at 1:1000 and competitor's antibody (sc-1851) diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

There are currently no references for ROCK2 antibody [GT11712] (GTX633389). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ROCK2 antibody [GT11712] (GTX633389). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations