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ROCK2 antibody [N3C1], Internal

Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247
Anti-ROCK2 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108247
Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247
Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247
Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247
Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247
Anti-ROCK2 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX108247

Cat No. GTX108247

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

HeLa , HepG2 , 293T , H1299 , NIH-3T3 , JC , rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037903

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247

GTX108247 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [N3C1], Internal (GTX108247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108247

GTX108247 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ROCK2(GTX108247) antibody at 1:200 dilution.

Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247

GTX108247 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
5% SDS PAGE
GTX108247 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247

GTX108247 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
5% SDS PAGE
GTX108247 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247

GTX108247 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal (GTX108247) diluted at 1:500 and competitor's antibody (sc-1851) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ROCK2 antibody [N3C1], Internal used in Western Blot (WB). GTX108247

GTX108247 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
5% SDS PAGE
GTX108247 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX108247

GTX108247 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human esophagous cancer, using ROCK2(GTX108247) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

ROCK2 antibody [N3C1], Internal Cat No. GTX108247

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 27-Oct-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : RMG1 cells, MCF7 cells
Amount used : 30μg
Blocking : 5% non fat milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 18Hr
Lane Description : Lane1: RMG1 cells. Lane2: MCF-7 cells.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169