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ROCK2 antibody

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Immunoprecipitation (IP). GTX102619
Anti-ROCK2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102619
Anti-ROCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

Cat No. GTX102619

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Rat2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , A549 , H1299 , HCT116

Predict Reactivity

Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:2000.

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody (GTX102619) diluted at 1:1000 and competitor's antibody (sc-1851) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ROCK2 antibody used in Immunoprecipitation (IP). GTX102619

GTX102619 IP Image

Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 μg of ROCK2 antibody (GTX102619).
Western blot analysis was performed using ROCK2 antibody (GTX102619).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ROCK2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102619

GTX102619 IHC-Fr Image

ROCK2 antibody detects ROCK2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-ROCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102619

GTX102619 ICC/IF Image

ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102619

GTX102619 IHC-P Image

ROCK2 antibody detects ROCK2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ROCK2 antibody (GTX102619) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at a dilution of 1:1000.

REFERENCE
REVIEW

ROCK2 antibody Cat No. GTX102619

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : atrial fibroblast cells
Amount used : 50μg
Blocking : 3% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169