APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Rat2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , A549 , H1299 , HCT116 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.4 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885235
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho associated coiled-coil containing protein kinase 2 , ROCK-II
Cellular Localization
Cytoplasm , Cell membrane; Peripheral membrane protein
Background
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102619 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:2000.
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GTX102619 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX102619 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody (GTX102619) diluted at 1:1000 and competitor's antibody (sc-1851) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX102619 ICC/IF Image
ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX102619 WB Image
ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000
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GTX102619 WB Image
ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000
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GTX102619 IHC-Fr Image
ROCK2 antibody detects ROCK2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX102619 IHC-P Image
ROCK2 antibody detects ROCK2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ROCK2 antibody (GTX102619) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102619 WB Image
ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000
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GTX102619 IP Image
Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 μg of ROCK2 antibody (GTX102619).
Western blot analysis was performed using ROCK2 antibody (GTX102619).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX102619 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
REVIEW
ROCK2 antibody
Cat. No. GTX102619
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 19-May-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
atrial fibroblast cells
Amount used :
50μg
Blocking :
3% milk in PBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
