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ROCK2 antibody

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Immunoprecipitation (IP). GTX102619
Anti-ROCK2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102619
Anti-ROCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619
Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102619
Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

Cat No. GTX102619

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Rat2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , A549 , H1299 , HCT116

Predict Reactivity

Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885235

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:2000.

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody (GTX102619) diluted at 1:1000 and competitor's antibody (sc-1851) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ROCK2 antibody used in Immunoprecipitation (IP). GTX102619

GTX102619 IP Image

Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 μg of ROCK2 antibody (GTX102619).
Western blot analysis was performed using ROCK2 antibody (GTX102619).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ROCK2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102619

GTX102619 IHC-Fr Image

ROCK2 antibody detects ROCK2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-ROCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102619

GTX102619 ICC/IF Image

ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102619

GTX102619 IHC-P Image

ROCK2 antibody detects ROCK2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ROCK2 antibody (GTX102619) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ROCK2 antibody used in Western Blot (WB). GTX102619

GTX102619 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at a dilution of 1:1000.

REFERENCE

REVIEW

ROCK2 antibody Cat No. GTX102619

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : atrial fibroblast cells
Amount used : 50μg
Blocking : 3% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169