Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:2000.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody (GTX102619) diluted at 1:1000 and competitor's antibody (sc-1851) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX102619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 μg of ROCK2 antibody (GTX102619).
Western blot analysis was performed using ROCK2 antibody (GTX102619).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

ROCK2 antibody detects ROCK2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ROCK2 antibody (GTX102619) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ROCK2 antibody detects ROCK2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

ROCK2 antibody detects ROCK2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
ROCK2 antibody (GTX102619) dilution: 1:1000

ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:500.
Blue: Hoechst 33342 staining.