Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at 1:4000.

ROCK2 antibody detects ROCK2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ROCK2 antibody (GTX122652) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at a dilution of 1:5000.

Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 μg of ROCK2 antibody (GTX122652).
Western blot analysis was performed using ROCK2 antibody (GTX122652) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 protein stained by ROCK2 antibody (GTX122652) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.