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ROCK2 antibody

Anti-ROCK2 antibody used in Western Blot (WB). GTX122652
Anti-ROCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122652
Anti-ROCK2 antibody used in Western Blot (WB). GTX122652
Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122652
Anti-ROCK2 antibody used in Immunoprecipitation (IP). GTX122652

Cat No. GTX122652

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ROCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167866

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROCK2 antibody used in Western Blot (WB). GTX122652

GTX122652 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at 1:4000.

Anti-ROCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122652

GTX122652 ICC/IF Image

ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 protein stained by ROCK2 antibody (GTX122652) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ROCK2 antibody used in Western Blot (WB). GTX122652

GTX122652 WB Image

ROCK2 antibody detects ROCK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody (GTX122652) diluted at a dilution of 1:5000.

Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122652

GTX122652 IHC-P Image

ROCK2 antibody detects ROCK2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ROCK2 antibody (GTX122652) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 antibody used in Immunoprecipitation (IP). GTX122652

GTX122652 IP Image

Immunoprecipitation of ROCK2 protein from HeLa whole cell extracts using 5 μg of ROCK2 antibody (GTX122652).
Western blot analysis was performed using ROCK2 antibody (GTX122652) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

There are currently no reviews for ROCK2 antibody (GTX122652). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169