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ROCK2 antibody

Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31837
Anti-ROCK2 antibody used in Western Blot (WB). GTX31837
Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31837

Cat. No. GTX31837

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ROCK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

Mouse Brain Tissue Lysate(GTX27934)

Product Note

Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ROCK2 antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2.The immunogen is located within amino acids 920 - 970 of ROCK2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm,Cell membrane,Nucleus

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31837

GTX31837 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31837 ROCK2 antibody.
Working concentration : 20 μg/ml

Anti-ROCK2 antibody used in Western Blot (WB). GTX31837

GTX31837 WB Image

WB analysis of mouse brain tissue lysate using GTX31837 ROCK2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-ROCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31837

GTX31837 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31837 ROCK2 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399