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ROCK2 (phospho Ser1366) antibody [HL1071]

Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103
Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636103
Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103

Cat No. GTX636103

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1071

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1366 of human ROCK2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909948

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm,Cell membrane,Nucleus

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103

GTX636103 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody [HL1071] (GTX636103) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636103

GTX636103 ICC/IF Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 (phospho Ser1366) stained by ROCK2 (phospho Ser1366) antibody (GTX636103) diluted at 1:500.

Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103

GTX636103 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody [HL1071] (GTX636103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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Package List Price ($)
$ 369
$ 169
Custom Conjugations