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ROCK2 (phospho Ser1366) antibody

Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651
Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

Cat No. GTX122651

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IA
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IA Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Positive Control

HeLa , 293T , 293T transfected with wild type (WT) plasmids

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human ROCK2 (phospho Ser1366). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2,ROCK-II

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651

GTX122651 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651

GTX122651 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:2000. (CIP: Calf-intestinal alkaline phosphatase)

Anti-ROCK2 (phospho Ser1366) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122651

GTX122651 ICC/IF Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 (phospho Ser1366) protein stained by ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 protein at nucleus and cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
ROCK2 (phospho Ser1366) antibody (GTX122651) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ROCK2 (phospho Ser1366) antibody used in Western Blot (WB). GTX122651

GTX122651 WB Image

ROCKII (phospho Ser1366) antibody  detects the phosphorylation of ROCKII at Ser 1366 residue in IP experiments.
HEK293T cells were transfected with wild type (WT) or phospho-mutant (S1366A) ROCKII expression constructs.
Overexpressed Myc-ROCKII proteins were immunoprecipitated with anti-myc antibody and probed with anti- ROCKII (phospho Ser1366) antibody (GTX122651, 1:1000 dilution) with or without peptide (0.2 mg/ml) competition as indicated.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 (phospho Ser1366) antibody used in IHC (Paraffin sections) (IHC-P). GTX122651

GTX122651 IHC-P Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 protein at weak nucleus and cytosol on human breast tissue by immunohistochemical analysis.
Sample: Paraffin-embedded human breast tissue.
ROCK2 (phospho Ser1366) antibody (GTX122651) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169