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ROR alpha antibody [C3], C-term

Anti-ROR alpha antibody [C3], C-term used in Western Blot (WB). GTX108201
Anti-ROR alpha antibody [C3], C-term used in Western Blot (WB). GTX108201
Anti-ROR alpha antibody [C3], C-term used in Western Blot (WB). GTX108201
Anti-ROR alpha antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108201

Cat. No. GTX108201

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all ROR alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

HeLa , mouse eye , mouse kidney

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ROR alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037906

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAR related orphan receptor A , IDDECA , NR1F1 , ROR1 , ROR2 , ROR3 , RZR-ALPHA , RZRA

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ROR alpha antibody [C3], C-term used in Western Blot (WB). GTX108201

GTX108201 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ROR alpha antibody [C3], C-term (GTX108201) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROR alpha antibody [C3], C-term used in Western Blot (WB). GTX108201

GTX108201 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ROR alpha antibody [C3], C-term (GTX108201) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROR alpha antibody [C3], C-term used in Western Blot (WB). GTX108201

GTX108201 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
ROR alpha antibody
GTX108201 diluted at 1:1000

Anti-ROR alpha antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108201

GTX108201 ICC/IF Image

ROR alpha antibody [C3], C-term detects ROR alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROR alpha stained by ROR alpha antibody [C3], C-term (GTX108201) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169